Immunohistochemical expression of P16 and cyclin D1 in oral lesions

Autores

  • José Ronildo Lins do Carmo Filho Universidade Federal do Ceará
  • Dennys Ramon de Melo Fernandes Universidade Federal do Ceará
  • Paulo Goberlânio Paulo Goberlânio de Barros Silva Universidade Federal do Ceará
  • Régia Maria do Socorro Vidal Patrocínio Universidade Federal do Ceará
  • Maria Elisa Quezado Lima Verde Universidade Federal do Ceará
  • Mário Rogério Mário Rogério Lima Mota Universidade Federal do Ceará
  • Fabrício Bitu Sousa Universidade Federal do Ceará
  • Ana Paula Negreiros Nunes Alves Universidade Federal do Ceará

DOI:

https://doi.org/10.59483/rfpp.v3n3.92

Palavras-chave:

Mouth Neoplasms, p16, Cyclin D1, Ki-67 Antigen

Resumo

Aim: To evaluate the immunohistochemical expression of p16, cyclin D1 and Ki67 in oral lesions (fibroepithelial hyperplasia, squamous papilloma and epidermoid carcinoma). Study type: This is a retrospective, quantitative and cross-sectional study, carried out by the survey of paraffin blocks in the Oral Pathology Laboratory of the Dentistry course of the Federal University of Ceará, from 2008 to 2014. Methods: A total of 89 samples were selected, grouped into: 25 fibroepithelialhyperplasias (FHE); 16 oral squamous papillomas (OP); 28 oral squamous cell carcinomas (SCC), subdivided between patients below 50 years (SCC-50) and above 50 years (SCC+50). Twenty cases of oral mucosa without microscopic changes were used as the control group (CTL). Main outcome measures: Morphological evaluation through hematoxylin-eosin staining; Quantitative and dichotomous immunoexpression by streptavidin-biotin technique (P16, cyclin D1 and Ki67). Results: All evaluated groups, except for SCC+50 and CTL, presented a significantly higher frequency of cytological changes consistent with koilocytosis (p <0.001). The benign oral lesions (FHE and OP) and the SCC-50 group showed high immunostaining for p16 and cyclin D1, being statistically significant in relation to the control group (p <0.001). The quantitative expression of Ki67 was higher in SCC-50 than in the CTL group, with statistical significance (p <0.001). Conclusion: Oral lesions (OP, FHE and SCC-50) showed, together, koilocytosis and significant positivity for p16 and cyclin D1. Further studies should be conducted to clarify the possible relationship between cytopathic changes and p16 expression.

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Publicado

27-09-2023

Como Citar

1.
Lins do Carmo Filho JR, de Melo Fernandes DR, Paulo Goberlânio de Barros Silva PG, do Socorro Vidal Patrocínio RM, Quezado Lima Verde ME, Mário Rogério Lima Mota MR, Bitu Sousa F, Negreiros Nunes Alves AP. Immunohistochemical expression of P16 and cyclin D1 in oral lesions. RFPP [Internet]. 27º de setembro de 2023 [citado 30º de maio de 2024];3(3). Disponível em: https://revistadeodontologia.facpp.edu.br/index.php/rfpp/article/view/92

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